石家庄学院学报

薄层色谱法(TLC)是一种快速分离和定性分析少量物质的常用实验技术,在原有薄层色谱法分离菠菜色素的实验中,所选择的展开剂中使用大量一类致癌物苯,实验过程中经常出现斑点拖尾、色素点分离不清晰等问题.为了避免使用致癌物苯且优化实验效果,选择非极性溶剂石油醚、正己烷、环己烷和极性溶剂乙醇、乙酸乙酯、丙酮多次组合进行薄层色谱分离菠菜色素实验,结果显示:当展开剂为正己烷-丙酮体积比为7:3时分离效果最好,胡萝卜素、脱镁叶绿素、叶绿素a、叶绿素b、叶黄素的R_f值分别为0.92,0.85,0.42,0.28,0.72,色素斑点完整、清晰度好.对该实验方法进行验证,结果显示其重现性和耐用性良好.

Thin layer chromatography(TLC) is an important experimental technique for rapid separation and qualitative analysis of a small amount of substances.In the original experiment of separating spinach pigment by TLC,the selected development agent uses a large amount of benzene,a carcinogen,and there usually occur spot tailing and unclear separation of pigment points during the experiment.In order to optimize the experimental effect,avoiding use of carcinogenic benzene,the non-polar solvent petroleum ether,n-hexane,cyclohexane and polar solvent ethanol,ethyl acetate and acetone have been combined for multiple times to separate spinach pigment by thin layer chromatography.The results show that when the volume ratio of n-hexane-acetone is 7:3,the separation effect is the best.When the Rf values of carotene,pheophytin,chlorophyll a,chlorophyll b and lutein are 0.92,0.85,0.42,0.28 and 0.72 respectively,and the pigment spots are complete and clarity.This method has been verified,and the result shows good reproducibility and durability.